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Pancreatic islet-like clusters from periosteum-derived progenitor cells

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Abstract

Recent studies comparing the insulin-producing cell (IPC) differentiation capacity of mesenchymal stem cells (MSCs) derived from four different sources (bone marrow, Wharton’s jelly, adipose tissue, and the periosteum) demonstrated that IPC differentiation of periosteum-derived progenitor cells (PDPCs) progressed faster than any other MSCs within 7 days, indicating that PDPCsare most suited to IPC differentiation. Here, two different cell culture methods, adhesion and cluster culture, were assessed for their ability to support in vitro IPC differentiation. The induction of IPC differentiation was confirmed by RTqPCR analysis of insulin gene expression levels and immunofluorescence analysis of insulin protein. An enzyme-linked immunosorbent assay was used to quantify secreted insulin. PDPC-derived IPCs from cluster cultures demonstrated a significantly increased expression of insulin and an enhanced secretion of insulin of insulin protein in response to glucose compared to IPCs derived from adhesion cultures. Thus, pancreatic islet-like cluster cultures appear to provide the optimal conditions such as cluster culture for IPC differentiation of PDPCs.

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Su-Jung Kim, Sang-Min Lim & Dong-Il Kim

  2. Department of Applied Bioscience, CHA University, Seongnam, 463-836, Korea

    Yong-Soo Choi & Sun-Mi Kim

  3. Lee Gil Ya Cancer and Diabetes Institute, College of Pharmacy, Gachon University, Incheon, 406-840, Korea

    Hee-Sook Jun, Eun-Young Park & Ok-Kyung Hwang

  4. Good Shepherd Hospital, Seoul, 135-080, Korea

    Chang-Woo Lee

Authors
  1. Su-Jung Kim
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  2. Yong-Soo Choi
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  3. Sun-Mi Kim
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  4. Sang-Min Lim
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  5. Hee-Sook Jun
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  6. Eun-Young Park
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  7. Ok-Kyung Hwang
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  8. Chang-Woo Lee
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  9. Dong-Il Kim
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Correspondence to Yong-Soo Choi or Dong-Il Kim.

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Kim, SJ., Choi, YS., Kim, SM. et al. Pancreatic islet-like clusters from periosteum-derived progenitor cells. Biotechnol Bioproc E 18, 1116–1121 (2013). https://doi.org/10.1007/s12257-013-0005-1

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  • DOI: https://doi.org/10.1007/s12257-013-0005-1

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