Skip to main content
SpringerLink
Log in

Chondrogenic properties of human periosteum-derived progenitor cells (PDPCs) embedded in a thermoreversible gelation polymer (TGP)

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Periosteum-derived progenitor cells (PDPCs) were isolated using a fluorescence-activated cell sorter and their chondrogenic potential in biomaterials was investigated for the treatment of defective articular cartilage as a cell therapy. The chondrogenesis of PDPCs was conducted in a thermoreversible gelation polymer (TGP), which is a block copolymer composed of temperature-responsive polymer blocks such as poly(N-isopropylacrylamide) and of hydrophilic polymer blocks such as polyethylene oxide, and a defined medium that contained transforming growth factor-β3 (TGF-β3). The PDPCs exhibited chondrogenic potential when cultured in TGP. As the PDPCs-TGP is an acceptable biocompatible complex appropriate for injection into humans, this product might be readily applied to minimize invasion in a defected knee.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lu, L., X. Zhu, R. G. Valenzuela, B. L. Currier, and M. J. Yaszemski (2001) Biodegradable polymer scaffolds for cartilage tissue engineering.Clin. Orthop. Relat. Res. 319S:S251-S270.

    Article  Google Scholar 

  2. Ochi, M., Y. Uchio, K. Kawasaki, S. Wakitani, and J. Iwasa (2002) Transplantation of cartilage-like tissue made by tissue engineering in the treatment of cartilage defects of the knee.J. Bone Joint Surg. Br. 84: 571–578.

    Article  CAS  Google Scholar 

  3. Kleinman, H. K., M. L. McGarvey, L. A. Liotta, P. G. Robey, K. Tryggvason, and G. R. Martin (1982) Isolation and characterization of type IV procollage, laminin, and heparan sulfate proteoglycan from the EHS sarcoma.Biochemistry 21: 6188–6193.

    Article  CAS  Google Scholar 

  4. Hishikawa, K., S. Miura, T. Marumo, H. Yoshioka, Y. Mori, T. Takato, and T. Fujita (2004) Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer.Biochem. Biophys. Res. Commun. 317: 1103–1107.

    Article  CAS  Google Scholar 

  5. Fisher, J. P., S. Jo, A. G. Mikos, and A. H. Reddi (2004) Thermoreversible hydrogel scaffolds for articular cartilage engineering.J. Biomed. Mater. Res. A 71: 268–274.

    Article  Google Scholar 

  6. Yoshioka, H., M. Mikami, and Y. Mori (1994) A synthetic hydrogel with thermoreversible gelation: I. Preparation and rheological properties.Pure Appl. Chem. A31: 113–120.

    Google Scholar 

  7. Nagaya, M., S. Kubota, N. Suzuki, M. Tadokoro, and K. Akashi (2004) Evaluation of thermoreversible gelation polymer for regeneration of focal liver injury.Eur. Surg. Res. 36: 95–103.

    Article  CAS  Google Scholar 

  8. De Bari, C., F. Dell'Accio, and F. P. Luyten (2001) Human periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age.Arthritis Rheum. 44: 85–95.

    Article  Google Scholar 

  9. O'Driscoll, S. W. and J. S. Fitzsimmons (2001) The role of periosteum in cartilage repair.Clin. Orthop. Relat. Res. 391S: S190-S207.

    Article  Google Scholar 

  10. Lim, S. M., Y. S. Choi, H. C. Shin, C. W. Lee and D.-I. Kim (2005) Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis.Biotechnol. Lett. 27: 607–611.

    Article  CAS  Google Scholar 

  11. Oh, I. S. and H. G. Kim (2004) Vascular endothelial growth factor upregulates follistatin in human umbilical vein endothelial cells.Biotechnol. Bioprocess Eng. 9: 201–206.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Engineering, Inha University, 402-751, Incheon, Korea

    Yong Soo Choi, Sang Min Lim, Hyun Chong Shin & Dong-II Kim

  2. Good Shepherd Hospital, 135-717, Seoul, Korea

    Chang Woo Lee

Authors
  1. Yong Soo Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sang Min Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hyun Chong Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chang Woo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dong-II Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dong-II Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, Y.S., Lim, S.M., Shin, H.C. et al. Chondrogenic properties of human periosteum-derived progenitor cells (PDPCs) embedded in a thermoreversible gelation polymer (TGP). Biotechnol Bioproc E 11, 550–552 (2006). https://doi.org/10.1007/BF02932082

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02932082

Keywords

Search

Navigation