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Effects of silkworm hemolymph and cartilage-specific extracellular matrices on chondrocytes and periosteum-derived progenitor cells

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Abstract

In order to enhance the repair of defects in articular cartilage via cell therapy with autologous chondrocytes, as well as with periosteum-derived progenitor cells (PDPCs), silkworm hemolymph (SH) and a variety of cartilage-specific extracellular matrices (ECMs) including type II collagen, proline, chondroitin 4-sulfate, and chondroitin 6-sulfate were assessed with regard to their efficacy as media supplements. SH, a known anti-apoptotic agent, was found to enhance cell growth, as was shown by the results of a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. According to the results of reverse transcriptase polymerase chain reaction (RT-PCR) analyses, the cartilage-specific ECMs were found to stimulate the expression of hyaline cartilage-specific genes, most notably type II collagen and Sox9, in monolayer cultures of PDPCs.

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, 402-751, Incheon, Korea

    Hyun Chong Shin, Yong Soo Choi, Sang Min Lim & Dong-Il Kim

  2. Good Shepherd Hospital, 135-717, Seoul, Korea

    Chang Woo Lee

Authors
  1. Hyun Chong Shin
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  2. Yong Soo Choi
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  3. Sang Min Lim
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  4. Chang Woo Lee
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  5. Dong-Il Kim
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Correspondence to Dong-Il Kim.

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Shin, H.C., Choi, Y.S., Lim, S.M. et al. Effects of silkworm hemolymph and cartilage-specific extracellular matrices on chondrocytes and periosteum-derived progenitor cells. Biotechnol. Bioprocess Eng. 11, 364–367 (2006). https://doi.org/10.1007/BF03026254

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  • DOI: https://doi.org/10.1007/BF03026254

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